Coding

Part:BBa_K4601010:Design

Designed by: Doriane Blaise   Group: iGEM23_Evry-Paris-Saclay   (2023-09-23)


NpSRII-NpHtrII-EnvZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 73
    Illegal NgoMIV site found at 331
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 801


Design Notes

Source

PCR amplifications from N. pharaonis and E. coli genomes, followed by Golden Gate assembly

References